Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v7i3.5443
Abstract
Objective: To investigate the functions of silk fibroin (SF) membranes with different ratios of dense layer to loose layer in preventing fibroblast infiltration and promoting bone marrow mesenchymal stem cell (BMSC) penetration, so as to provide experimental evidence and guidance for the design of clinical guided bone regeneration membranes. Methods: SF membranes with dense-to-loose layer ratios of 1:0, 1:1, 1:2, 1:4 and 0:1 were prepared. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy were used to characterize the material properties. Fluorescence staining was applied to detect the barrier effect of the membranes on fibroblasts and the guidance effect on BMSCs. A rat skull defect model with membrane implantation was established, and bone volume fraction was measured to evaluate the bone regeneration efficacy. Results: SEM revealed that the membrane exhibited an asymmetric structure with one dense side and one porous side. The penetration depths of fibroblasts in the 1:0, 1:1, 1:2 and 1:4 groups were significantly lower than those in the 0:1 group. The penetration depths of BMSCs in the 1:2, 1:4 and 0:1 groups were significantly higher than those in the 1:0 group. The rat skull defect experiment showed that the bone volume fractions in the 1:2 and 1:4 groups were higher than those in other groups. Conclusion: When the ratio of dense-to-loose layer of SF membrane is designed between 1:2 and 1:4, it can simultaneously achieve favorable barrier function and stem cell guidance ability, thereby effectively promoting bone regeneration.
Keywords
silk fibroin, guided bone regeneration, bone defect
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[18] Xie J, Shen H, Yuan G, et al. The effects of alignment and diameter of electrospun fibers on the cellular behaviors and osteogenesis of BMSCs[J]. Mater Sci Eng C Mater Biol Appl, 2021, 120:111787.
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