Research on the Anti-tumor Mechanism of Natural Antimicrobial Peptide TA
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v7i1.5056
Abstract
Natural antimicrobial peptides exhibit broad-spectrum antibacterial properties and the capacity to selectively eradicate tumour cells, presenting a promising avenue for the development of innovative anti-cancer medications[1,2]. Among these peptides, TA is a significant natural antimicrobial peptide currently undergoing investigation for its anti-tumour efficacy. This investigation focuses on human non-small cell lung cancer cells (A549 cells) as the experimental model. Cell morphology will be examined using scanning electron microscopy, while the mitochondrial membrane potential changes in A549 cells following various drug treatments will be monitored in real-time using the mitochondrial membrane potential probe DiSC3(5). The overall cell count and apoptotic cell distribution will be assessed alongside DAPI/PI double staining to elucidate the mechanism and effectiveness of TA in inducing cell death in A549 cells. Intriguingly, our experiments have revealed that TA can trigger the apoptotic pathway by disrupting the mitochondrial membrane potential of A549 cells, demonstrating significantly greater cytotoxicity compared to 5-FU and PT compounds. These findings provide empirical support for considering TA as a potential therapeutic option for lung cancer and propose a novel strategy for targeted therapy of non-small cell lung cancer.
Keywords
natural antimicrobial peptide TA; anti-tumor mechanism of action; cell apoptosis
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[4] Chengfei H,Quan W,Shuhui H, et al.Corrigendum: Gramicidin-S-Inspired Cyclopeptidomimetics as Potent Membrane-Active Bactericidal Agents with Therapeutic Potential.[J].ChemMedChem,2022,17(3):e202100765-e202100765.
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