Effects of Huanglian Ointment on Wound Healing in Mice with Deep Second-Degree Burns and the Related Mechanism
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v6i4.4626
Abstract
Objective: To investigate the effect of Huanglian ointment on wound healing in mice with deep second-degree burns and its potential mechanism. Methods: Sixty BALB/c mice were randomly divided into a Huanglian ointment group and a Vaseline group (control). A deep second-degree burn model was established on the dorsal skin. After modeling, the wounds of the two groups were covered with Huanglian ointment gauze and Vaseline gauze, respectively, with daily dressing changes. Wound healing was observed on days 0, 3, 7, and 17 after injury, and the eschar dissolution rate and healing rate were calculated. Wound tissues were collected at each time point for histopathological examination by H&E staining. ELISA was used to determine the levels of inflammatory factors (TNF-α, IL-1β, IL-6) and growth factors (VEGF, bFGF) in the wound tissues. Results: Compared with the Vaseline group, the Huanglian ointment group showed faster eschar dissolution and wound healing. On day 7 after injury, the eschar dissolution rate (22.36±0.95)% was significantly higher than that of the control group (12.69±0.47)%. On day 17 after injury, the healing rate (93.19±4.53)% was also significantly higher than that of the control group (74.54±3.37)%. Histological analysis showed reduced inflammatory cell infiltration and increased, more orderly arranged neovascularization and collagen formation in the Huanglian ointment group at days 7 and 17. ELISA results indicated that the levels of TNF-α, IL-1β, and IL-6 were significantly decreased, while VEGF and bFGF levels were significantly increased in the Huanglian ointment group on day 7 after injury. Conclusion: Huanglian ointment can effectively promote wound healing in mice with deep second-degree burns, and its mechanism may be related to inhibiting inflammatory responses and upregulating the expression of growth factors.
Keywords
Huanglian ointment; deep second-degree burns; wound healing
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[6] Xiao Changshuan, Liu Yaping, Sun Kui, Yang Jingzhe, Chen Hongyu. Intervention effect and mechanism of compound Huangbai liquid preparation on deep second-degree burn wounds [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2021, 16: 102-110.
[7] Ding Xiaotong, Wang Yiwei, Min Wen, Chen Jun. Therapeutic effect of Macrolepiota spore ethanol extract on full-thickness skin defect wounds in mice [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2022, 08: 149-158.
[8] Zhao Qiansi, Wang Xiong, Wu Jinhu. Research progress on the mechanisms of topical Chinese medicine promoting burn wound healing [J]. Medical Review, 2014, 16: 3003-3005.
[9] Ding Ling, Chen Zhiwei. Research progress on the mechanisms of topical Chinese medicine promoting wound healing [J]. Jiangsu Traditional Chinese Medicine, 2006, 12: 71-72.
[10] Xie Longwei, Gu Zaiqiu, Cai Liangliang. Clinical observation of Huanglian ointment in the treatment of early burns in 41 cases [J]. Henan Traditional Chinese Medicine, 2014, 12: 2487-2488.
[11] Meng Yazhi, Zhang Jing, Li Jun. Clinical observation of compound Huanglian ointment in the treatment of diaper dermatitis in children [J]. Modern Journal of Integrated Chinese and Western Medicine, 1999, 03: 465.
[12] Duan Yanfang. Treatment of second-degree burns in 200 cases using Huanglian gauze strips [J]. Hubei Journal of Traditional Chinese Medicine, 1996, 02: 41.
[13] Ji Guangqiong, Deng Yanqing, Tan Faming, Chen Zhiguo. Research progress of Huanglian ointment [J]. Hubei Journal of Traditional Chinese Medicine, 2018, 03: 55-57.
[14] Kunimura K, Miki S, Takashima M, et al. S-1-propenylcysteine improves TNF-α-induced vascular endothelial barrier dysfunction by suppressing the GEF-H1/RhoA/Rac pathway [J]. Cell Commun Signal, 2021, 19(1): 1-14.
[15] Alhilali M J, Parham A, Attaranzadeh A, et al. Prognostic role of follicular fluid tumor necrosis factor alpha in the risk of early ovarian hyperstimulation syndrome [J]. BMC Pregnancy Childbirth, 2020, 4(20): 1-7.
[16] Abali A E, Karakayali H, Ozdemir B H, et al. Destructive pulmonary effects of smoke inhalation and simultaneous alterations in circulating IL-6, TNF-α, and IFN-γ levels at different burn depths: an experimental study on rats [J]. J Burn Care Res, 2013, 34(3): 334-341.
[17] Losi P, Briganti E, Errico C, et al. Fibrin-based scaffold incorporating VEGF- and bFGF-loaded nanoparticles stimulates wound healing in diabetic mice [J]. Acta Biomaterialia, 2013, 9(8): 7814-7821. DOI:10.1016/j.actbio.2013.04.019.
[18] Gerwins P, Sköldenberg E, Claesson-Welsh L. Function of fibroblast growth factors and vascular endothelial growth factors and their receptors in angiogenesis [J]. Critical Reviews in Oncology and Hematology, 2000, 34(3): 185-194. DOI:10.1016/S1040-8428(00)00062-7.
[19] Yin Lianghong, Gao Ling, Lu Jidong, et al. Research progress on the role of basic fibroblast growth factor in skin injury repair [J]. Chinese Journal of Biological Products, 2020, 33(05): 574-580. DOI:10.13200/j.cnki.cjb.002628.
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