SCiRS-7 Enhances the Resistance of Endometrial Cancer Cells to Endoplasmic Reticulum Stress by Inhibiting the Expression of miR-7
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v6i2.4061
Abstract
Objective: To investigate the relationship between circular RNA ciRS-7 and endoplasmic reticulum (ER) stress in endometrial cancer (EC) cells. Methods: Stable ciRS-7-overexpressing Ishikawa EC cells were constructed via lentiviraltransfection. Proliferation, migration, and invasion were assessed via colony formation, scratch, and Transwell assays. MiR-7 levels and ER stress/apoptosis-related proteins were analyzed by qRT-PCR and Western blot. Overexpression/knockdown models were used to validate mechanisms. Results: ciRS-7 overexpression enhanced proliferation, migration, invasion, and ER stress resistance by suppressing miR-7, reducing pro-apoptotic proteins. Dual ciRS-7/miR-7 overexpression or ciRS-7 knockdown reversed these effects, increasing apoptosis and ER stress sensitivity. Conclusion: ciRS-7 promotes ECprogression and ER stress resistance via miR-7 inhibition.
Keywords
SCiRS-7, endometrial cancer, miR-7
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