Research Progress and Advantages of RNA Bridge Gene Editing Technology
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v6i1.3660
Abstract
The purpose of this paper is to deeply discuss the principle, application, and comparative advantages of gene editing technology, especially RNA bridge gene editing technology. Firstly, the background and significance of the development of gene editing technology were introduced, and then the principle and application fields of RNA bridge gene editing technology were elaborated, including the achievements in disease treatment and agriculture. Then, the principles, challenges, and applications of traditional gene editing technologies (ZFN, TALEN, CRISPR-Cas9) are summarized. Through comparison, the advantages of RNA bridge gene editing technology compared with traditional technology, such as high specificity, high editing efficiency and strong flexibility, were analyzed. Finally, this paper summarizes and prospects the RNA bridge gene editing technology, and points out that although it has potential, it still needs to be further studied on its feasibility, safety, efficiency, and specificity in different species and cell types.
Keywords
gene editing technology; RNA bridges; traditional techniques; Comparison of advantages
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[5]Khalil AM. The genome editing revolution: review. J Genet Eng Biotechnol. 2020;18(1):68.
[6]Zhang HX, Zhang Y, Yin H. Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9. Mol Ther. 2019;27(4):735-746.
[7]Bacman SR, Kauppila JHK, Pereira CV, et al. MitoTALEN reduces mutant mtDNA load and restores tRNA(Ala) levels in a mouse model of heteroplasmic mtDNA mutation. Nat Med. 2018;24(11):1696-1700.
[8]NI Peiling, LIU Chang, CHEN Lingyi. DNA scissors — TALEN和CRISPR/Cas. Chinese Journal of Cell Biology. 2014; 36(01):5-11.
[9]Janik E, Niemcewicz M, Ceremuga M, et al. Various Aspects of a Gene Editing System-CRISPR-Cas9. Int J Mol Sci. 2020;21(24).
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