Hemin Downregulates the Effect of GPX4 on the Malignant Biological Behavior of Bladder Cancer T24 Cells

Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v5i3.2789

Zikai Tang, Qingze Xiao, Wei Xiao

Department of Urology, Hunan Province Peopel’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410000, Hunan, China

Abstract

In a study of curcumin's effects on bladder cancer, T24 cells were used. Cells were divided into a control and 3 curcumin-treated groups (10, 20, 40 μmol/L). CCK8 showed curcumin inhibited proliferation in a time- and concentration-dependent manner. Flow cytometry showed increased apoptosis in curcumin-treated cells. Scratch test and Transwell assay indicated reduced migration and invasion. Western Blot revealed upregulation of HMOX1 and downregulation of GPX4 proteins in curcumin-treated groups (P<0.05). These findings suggest curcumin exerts antitumor effects by inhibiting proliferation, migration, invasion, and inducing apoptosis through downregulation of GPX4 pathway.

Keywords

bladder cancer; malignant evolution; Hemin; GPX4

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