Feasibility Study on Using Syringes to Separate Human Peripheral Blood for Preparing Platelet-Rich Plasma (PRP)
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v5i2.2291
Abstract
This paper focuses on exploring new methods for preparing platelet-rich plasma (PRP) to improve its clinical application and popularization. As a natural growth factor, PRP has significant advantages in promoting tissue repair and alleviating inflammation, but limitations in preparation methods hinder its widespread use. The study uses clinical medical syringes as the centrifuge container for PRP preparation. Without disrupting the stratification of the blood, the method employs a pushing technique to move the density gradient centrifuged blood through a medical three-way valve, separating the different blood components to obtain the target PRP layer. This method is simple to operate, has a higher recovery efficiency, and ensures the blood is handled in a closed environment throughout the process, reducing environmental contamination. It can be performed in ordinary settings. The authors seek a feasible, easy-to-operate, environmentally low-requirement, and low-cost separation method to facilitate the clinical popularization of PRP.
Keywords
PRP (platelet-rich plasma), PRP preparation, syringe as PRP centrifuge container, pushing technique, separation of blood components
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[1]Wang LL (Ed.). Clinical Immunology and Immunological Testing, a compulsory course guide for medical laboratory professionals. Science and Technology Literature Publishing House, October 2004, 1st Edition, p. 111.
[2]Zhu XF. Medical Conventional Testing Instruments. Science Press, 2014.
[3]Retrieved from https://baike.so.com/doc/6936828-7159184.html, last updated by ffuo5825, September 25, 2020.
[4]Tian SY, Yang XM. Production and Clinical Application of Platelet-Rich Plasma (PRP). 2021.
[5]Wang SJ, Wen CJ, Li SY. Comparison of Cell and Cytokine Components in Platelet-Rich Plasma (PRP) Prepared by Different Kits. Chinese Journal of Orthopedics: Electronic Edition, 2016, 10(6): 5. DOI: 10.3877/cma.j.issn.1674-134X.2016.06.002.
[6]Kim G, Jeong S, Yang JK. Ultrasound Standing Wave-Based Cell-to-liquid Separation for Measuring Viscosity and Aggregation of Blood Sample. Sensors, 2020, 20(8):2284.
[7]Yu Z, Yong KA, Fu J. Microfluidic Blood Cell Sorting: Now and Beyond. Small, 2014, 10(9):1687-1703.
[8]Hojin Kim, Alexander Zhbanov, Sung Yang. Microfluidic Systems for Blood and Blood Cell Characterization. Biosensors 2023, 13(1): 13. https://doi.org/10.3390/bios13010013.
[2]Zhu XF. Medical Conventional Testing Instruments. Science Press, 2014.
[3]Retrieved from https://baike.so.com/doc/6936828-7159184.html, last updated by ffuo5825, September 25, 2020.
[4]Tian SY, Yang XM. Production and Clinical Application of Platelet-Rich Plasma (PRP). 2021.
[5]Wang SJ, Wen CJ, Li SY. Comparison of Cell and Cytokine Components in Platelet-Rich Plasma (PRP) Prepared by Different Kits. Chinese Journal of Orthopedics: Electronic Edition, 2016, 10(6): 5. DOI: 10.3877/cma.j.issn.1674-134X.2016.06.002.
[6]Kim G, Jeong S, Yang JK. Ultrasound Standing Wave-Based Cell-to-liquid Separation for Measuring Viscosity and Aggregation of Blood Sample. Sensors, 2020, 20(8):2284.
[7]Yu Z, Yong KA, Fu J. Microfluidic Blood Cell Sorting: Now and Beyond. Small, 2014, 10(9):1687-1703.
[8]Hojin Kim, Alexander Zhbanov, Sung Yang. Microfluidic Systems for Blood and Blood Cell Characterization. Biosensors 2023, 13(1): 13. https://doi.org/10.3390/bios13010013.
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