Study on Paeoniflorin Promoting the Recovery of Motor Function of Rat Spinal Cord Injury by Mediating MAPK/ERK and Akt/mTOR Signal Pathway

Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v2i1.302

Pei Zhang¹, Nan Wu², Zhijun Song³, Zhengfu Tai⁴

1. School of Chemical Engineering, Yunnan Open University
2. School of Medical, Yunnan University of Business Management
3. National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants
4. Sichuan Kelun Pharmaceutical Research Institute

Abstract

Spinal cord injury (SCI) is a devastating disease that can cause severe motor, sensory, and autonomic dysfunction. There is currently no effective treatment. Paeonia lactiflora is a traditional Chinese herbal medicine, which has antispasmodic, analgesic, and blood circulation effects. Paeoniflorin (PEF) is a medicinal plant isolated from Paeoniae Radio, and it is widely used in East Asia. A large number of studies have shown that PEF has a powerful neuroprotective effect. However, the potential mechanism of PEF on SCI needs further study. This project uses Basso Beattie Bresnahan (BBB) motor function score and open field test to evaluate neurological function, and uses immunofluorescence method to detect brain-derived neurotrophic factor (BNDF) and neurotrophin-3 (NT). -3) Protein expression, Western blot is used to detect protein expression level, and RT-PCR is used to detect mRNA expression level. Thus, a rat spinal cord injury model was established to observe the effects of AT/mTOR and MAPK/ERK signal pathways activated by PEF on nerve regeneration and functional recovery in rats with spinal cord injury.

Keywords

spinal cord injury, paeoniflorin, recovery

Funding

"Paeoniflorin promotes the recovery of motor function after spinal cord injury in rats" in Yunnan Open University (No.: 19YNOU05)

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