不同类型电子供体驱动的反硝化脱氮效果与机制
Journal: Ecological Environment and Protection DOI: 10.32629/eep.v9i3.3122
Abstract
随着水污染问题的日益严重和生态环境保护的迫切需要,污水中硝态氮去除效率的提 升迫在眉睫且已成为氮去除的主要瓶颈。本研究通过添加有机电子供体和无机电子 供体分别促发自养反硝化和异养反硝化过程对比两种类型电子供体对硝态氮去除效 率的影响。结果表明,有机电子供体通过显著提升反硝化全流程功能基因(如narG、 nirK、nosZ)丰度,实现了更为高效的反硝化速率和硝态氮去除效率(50%以上),其持 续电子供给能力及碳源特性驱动了有机碳降解相关微生物群落的演替。无机电子供 体虽可激活反硝化前期基因表达,但因电子供应间断性和氧化微环境导致反硝化过程 的不彻底,最终导致亚硝态氮积累,反硝化速率和硝态氮去除效率明显低于有机电子 供体实验组,但部分无机电子供体的氧化过程可驱动磷的化学吸附(磷去除效率提升 20%以上),表现出氮磷协同去除特性。无机电子供体的添加显著富集氧化菌的功能菌 群。因此,有机供体仍是当前强化反硝化脱氮的优选策略,而无机供体需通过优化电 子传递体系提升反硝化过程的完整性,其多污染协同去除特性为废水处理提供了新的 技术方向。
Keywords
有机电子供体；无机电子供体；反硝化
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[4] Wick,K.,Heumesser, C. and Schmid, E. (2012) Groundwater nitrate contamination:Factors and indicators.Journal of Envir onmental Management111,178-186.
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[9] Murphy,J.and Riley,J.P.(1962)A modified single soluti on method for the determination of phosphate in natural wat ers.Analytica Chimica Acta 27,31-36.
[10] 国家环保局本书编委会.水和废水监测分析方法[M].中国环境科学出版社,1989.
[11] Risgaard-Petersen,N.,Nielsen,L.P.,Rysgaard,S.,Dalsgaard,T.,Meyer, R.L.J.L. and Methods, O. (2003) Application of the isotope pairing technique in sediments where anammox and denitrifica tion coexist.Limnology and Oceanography:Methods 63-73.
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[23] Fan,Z.,Hu,J.and Wang,J.(2012)Biological nitrate remov al using wheat straw and PLA as substrate. Environ Technol 33(19-21),2369- 2374.
[24] Liang,J.,Chen,N.,Tong,S.,Liu, Y.and Feng,C.(2018)Sulfur autotrophic denitrification(SAD)driven by homogeneous compo site particles containing CaCO3-type kitchen waste for groun dwater remediation.Chemosphere 212,954-963.
[25] Li,Y.,Liu,L.and Wang,H.(2022)Mixotrophic denitrificati on for enhancing nitrogen removal of municipal tailwater: Contribution of heterotrophic/sulfur autotrophic denitrifica tion and bacterial community. Science of The Total Environme nt 814,151940.
[26] Haroun,B.,El-Qelish,M.,Kianizadeh,S.,Muller,C.,Bronstad, E., Oza,S.,Kakar, F., Bell, K.Y., Wells, M.J.M. and Nakhla, G. (2025) Long term impact of lignin accumulation in cattle manure dige sters on biomass activity and chemical post treatment. Energy Conversion and Management 327,119581.
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