Journal: Agricultural Science DOI: 10.32629/as.v9i7.4182
Abstract
为探究不依赖组培的豌豆遗传转化方法,以中豌六号豌豆品种为材料,利用含不同发 育调节因子的载体,通过农杆菌(Agrobacterium tumefaciens)直接注射递送法侵染植株,以PCR检测再生植株确认阳性。结果表明： 不同发育调节因子对再生和转化效率影响较大,AtGRF5和ZmWUS+ipt介导的再生效率 最高(均>50%),TaGRF4- OsGIF1介导的转化效率最高(29.9%)。农杆菌浓度也影响再生和转化效率,OD600=0. 8时两者均明显提升。本研究表明,基于发育调节因子的直接注射递送可有效介导豌 豆的再生和转化,可为豌豆功能基因的分子生物学研究提供参考。
Keywords
豌豆；直接递送法；遗传转化；发育调控因子
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[15] Maher,M.F.,Nasti, R.A.,Vollbrecht, M.,Starker,C.G.,Clark,M.D.Voytas,D.F.Plant Gene Editing through De Novo Induction of Meristems[J].Nat Biotechnol,2020,38,84-89.
[16] 刘之洋,刘静,宁宇,等.基于fast- Tracc方式建立苦瓜高效遗传转化体系的研究[J].热带生物学报,2023,(4):622-627.
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