蝉花虫草 N 6-(2-羟乙基)腺苷的提取及神经保护作用
Journal: Agricultural Science DOI: 10.32629/as.v9i5.3979
Abstract
随着全球人口老龄化进程的加速,阿尔茨海默症等神经退行性疾病严重威胁人类健康 ,现有治疗药物十分有限且副作用明显。天然产物是发现新药先导化合物的重要来源 ,而蝉花虫草为我国传统名贵中药材,其有效成分N⁶-(2- 羟乙基)腺苷是一种特征性嘌呤核苷衍生物。研究表明,N⁶-(2- 羟乙基)腺苷展现出多靶点协同干预神经病理环节的保护作用,具有成为抗神经退行 性疾病药物先导化合物或功能食品因子的重要潜力,深入研究其机制对开发新型神经 疾病治疗策略具有科学意义。
Keywords
蝉花虫草；N⁶-(2-羟乙基)腺苷；提取工艺；神经保护作用
Funding
(1)辽宁省教育厅本科高校基本科研项目平台建设专项(LJ232510166004)；(2)辽宁省科技计划联合计划项目(2025-BSLH-372)；(3)沈阳师范大学博士、引进人才科研项目启动基金项目(BS202526)。
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[2] 徐红娟,肖楠,王玉方,等.蝉花的研究进展[J].中国药业,2010,19(17):85-87.
[3] OlatunjiOJ,TangJ,TolaA,etal.NeuroprotectiveeffectsofCordycepsspecies:ar eview[J].Phytomedicine,2016,23(S1):S46-S54.
[4] 陈丽萍,赵杰宏,王娇,等.蝉花预防治疗阿尔兹海默症的网络药理学分析[J].山地农 业生物学报,2024,43(4):11-17,38.
[5] FuruyaT,HirotaniM,Matsuzawa M.N6-(2-hydroxyethyl) adenosine,a biologically active compound from cultured myce lia of Cordyceps and Isaria species[J].Phytochemistry,1983,22 (11):2509-2512.
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[8] 长城,秦立武.响应面法优化细脚棒束孢中N6-(2- 羟乙基)腺苷的超声提取工艺研究[J].菌物学报,2019,38(9):1559-1569.
[9] LiuKB,WangF,Wang W Z, et al. Beauveria bassiana: a new N6-(2- hydroxyethyl)-adenosine-producing fungus[J].Journal of Asian Natural Products Research,2017,19(12):1219-1227.
[10] 雷帮星,何劲,康冀川.细脚棒束孢E3菌株产N6-(2- 羟乙基)腺苷的培养基及前体物筛选[J].食用菌学报,2013,20(2):46-51.
[11] 武晨科.N6-(2- 羟乙基)腺苷的制备策略与药用潜力研究综述[J].化工生产与技术,2025,31(5):9- 12,24,61-62.
[12] 艾仁丽,谭艾娟,吕世明.蝉花中N6-(2-羟乙基)- 腺苷提取工艺优化及结构鉴定[J].食用菌学报,2023,30(2):65-74.
[13] 张忠,周帅,刘艳芳.高速逆流色谱分离制备蛹虫草中虫草素和N6-(2-羟乙基)- 腺苷[J].菌物学报,2016,35(6):742-749.
[14] 雷帮星,何劲,康冀川,等.细脚棒束孢E3菌株产N6-(2- 羟乙基)腺苷的液体发酵条件优化[J].菌物研究,2014,12(2):96-100.
[15] 廖兴刚,孟泽彬.粉被虫草液体静置发酵产N6-(2- 羟乙基)腺苷培养基优化[J].分子植物育种,2025,23(9):3046-3051.
[16] 刘宽博.产N6-(2- 羟乙基)腺苷(HEA)的虫草菌资源及HEA生物合成调控研究[D].贵阳:贵州大学,2018.
[17] QuGZ,Li J, Guo Y H,et al. Microwave-assisted synthesis of N6-(2- hydroxyethyl)adenosine and its analogues[J].Synthe tic Communications,2006,36(18):2647-2656.
[18] 王冬梅,张娟,杨海涛,等.N6-(2- 羟乙基)腺苷的合成研究[J].化学试剂,2013,35(1):53-55.
[19] 朱碧纯,柴一秋,章思思,等.蝉花虫草活性成分的抗惊厥作用[J].菌物学报,2016, 35(5):619-627.
[20] LuMY,Chen C C,Lee L Y,et al.N6-(2-hydroxyethyl)adeno sine in the medicinal mushroom Cordyceps cicadae attenuates lipopolysaccharide-stimulated pro-inflammatory responses by suppressing TLR4-mediated NF-κB signaling pathways[J]. Journal of Natural Products,2015,78(10):2452-2460.
[21] Zhang L,Wu T,Olatunji O J,et al.N6-(2-hydroxyethyl) -adenosine from Cordyceps cicadae attenuates hydrogen pero xide induced oxidative toxicity in PC12 cells[J].Metabolic Br ain Disease,2019,34(5):1325-1334.
[22] Wu Y Z,Lee C L.Cordyceps cicadae NTTU 868 mycelium with the addition of bioavailable forms of magnesium from deep ocean water prevents the Aβ40 and streptozotocin-indu ced memory deficit via suppressing Alzheimer's disease risk factors and increasing magnesium uptake of brain[J]. Ferment ation,2021,7(1): 39.
[23] Chang C Y, Yang P X, Yu T L, et al. Cordyceps cicadae NTTU 868 mycelia fermented with deep ocean water minerals prevents D-galactose- induced memory deficits by inhibiting oxidative inflammatory factors and aging-related risk facto rs[J].Nutrients,2023,15(8):1968.
[24] 凌春燕.基于秀丽隐杆线虫模型的N6-(2- 羟乙基)腺苷抗阿尔兹海默症活性研究[D].杭州:中国计量大学,2024.
[25] 王冬梅,刘晓辉,郭辉,等.腺苷类似物的设计、合成及活性研究[J].药学学报,201 3,48(6):881-886.
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