二苯乙烯苷对同型半胱氨酸诱导的血管功能损伤的保护作用
Journal: Journal of Rural Medicine DOI: 10.12238/jrm.v1i2.11489
Abstract
目的：观察二苯乙烯苷对同型半胱氨酸诱导的血管损伤的保护作用。方法：采用离体器官段培养方法，以同型半胱氨酸和不同浓度的二苯乙烯苷共同处理血管段，用DMT Myograph动描计系统记录在K+、PE、5-HT缩血管物质的刺激下离体血管张力的变化；建立高同型半胱氨酸血症(HHcy)小鼠动物模型，随机分为对照组、HHcy模型组、二苯乙烯苷低剂量组(40mg/kg)、二苯乙烯苷中剂量组(80mg/kg)、二苯乙烯苷高剂量组(160mg/kg)共5组。治疗结束后，ELISA法检测血中AngⅡ、AD、NE、5-HT水平；病理组织学检查各组小鼠主动脉和微动脉血管形态结构、血管平滑肌纤维及胶原蛋白沉积情况。结果：不同浓度的二苯乙烯苷可显著抑制体外培养的血管平滑肌对缩血管物质的敏感性；二苯乙烯苷可降低HHcy模型小鼠血中AngⅡ的水平；二苯乙烯苷给药后，模型组小鼠主动脉、微动脉血管胶原纤维沉积减少，微动脉血管的弹力纤维增加。结论：二苯乙烯苷可降低Hcy引起血管平滑肌对缩血管物质的敏感性，抑制此类物质对血管的收缩作用，降低HHcy模型小鼠AngⅡ水平，还可改善HHcy引起的微动脉损伤，且其对微动脉的保护作用明显强于输送血管。
Keywords
二苯乙烯苷；同型半胱氨酸；血管平滑肌；血管损伤
Funding
陕西省科技厅项目2023-JC-YB-753；陕西省教育厅重点实验室22JS038；西安医学院校级科技创新团队2021TD06。
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[15] Hieronim, Jakubowski. Homocysteine Modification in Protein Structure/Function and Human Disease[J]. Physiol Rev. 2019,99(1):555-604.
[16] Kaplan P,Tatarkova Z,Sivonova MK,et al.Homocysteine and Mitochondria in Cardiovascular and Cerebrovascular Syst ems[J].Int J Mol Sci.2020,18;21(20):7698.
[17] Fang P, Zhang D, Cheng Z, et al. Hyperhomocysteinemia Potentiates Hyperglycemia-Induced Inflammatory Monocyte Differentiation and Atherosclerosis[J].Diabetes. 2014. 63(12): 4275-4290.
[18] Tan H, Shi C, Jiang X, et al. Hyperhomocysteinemia promotes vascular remodeling and fibrosis in carotid vein graph in mice[J].Front Biosci (Landmark Ed).2014,19: 958-966.
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[22] Zhu Z,Li S,Jia Y,et al.Homocysteine promotes migration of adventitial fibroblasts via angiotensin II type 1 receptor activation to aggravate atherosclerosis[J].Gen Physiol Bioph ys.2022,41(6):535-547
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