内毒素加/不加二甲基肼复制大肠腺瘤模型的比较
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v7i6.17042
Abstract
目的：采用内毒素加/不加二甲基肼复制大肠腺瘤模型并比较其异同。方法：14只约210g雄性Wistar大鼠分为正常组与3个模型组：单纯长期尾静脉注射内毒素（模型Ⅰ组）、长期尾静脉注射内毒素加腹腔注射二甲基肼（模型Ⅱ组）、内毒素加二甲基肼与GW9662（模型Ⅲ组），检测血清内毒素水平与炎症因子等，实验到期后取肠管等，肉眼及光镜下观察。结果：模型Ⅰ、Ⅱ与Ⅲ组血清内毒素与炎症因子均升高，均成功复制大肠腺瘤，模型Ⅰ为凹陷型管状腺瘤，瘤组织向粘膜下浸润，模型Ⅱ为扁平型管状腺瘤，模型Ⅲ为隆起型绒毛状管状腺瘤。结论：模型Ⅰ、Ⅱ与Ⅲ组复制的大肠腺瘤肉眼与光镜下病理分型不同，应该与毒素种类、注射剂量及作用时间等有关。
Keywords
内毒素；尾静脉注射；1，2-二甲基肼；腹腔注射；大肠腺瘤
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