TRPC1通道蛋白在龋坏牙髓组织中的表达
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v7i6.17013
Abstract
目的成牙本质细胞通过TRP通道蛋白发挥了接收和传递刺激以及修复的作用，TRP通道蛋白是一类具有阳离子渗透性的超家族离子通道蛋白，其中TRPC1可能参与了修复性牙本质的形成，本实验的目的是研究龋坏对成牙本质细胞中TRPC1表达的影响。方法用HE染色检测龋坏牙齿切片的形态，采用免疫组化方法研究TRPC1在健康和龋坏牙髓组织中的表达。结果HE染色结果显示切片质量良好，符合后续实验要求，免疫组化分析表明在健康人牙髓细胞中检测到TRPC1的表达，且在成牙本质细胞中呈现高表达特征。此外，随着龋病的进展，TRPC1的表达水平呈增高趋势。结论综上所述，本研究表明TRPC1表达水平受牙齿龋坏的影响，其可能参与修复性牙本质的形成。
Keywords
成牙本质细胞；龋坏；TRPC1通道蛋白；修复性牙本质
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[1] 孟润莎,徐琼.成牙本质细胞在牙髓免疫防御中的作用研究进展[J].中华口腔医学研究杂志(电子版),2017,11(4):238-41.
[2] 郭京,单徜,李玫,等.TRP离子通道在干细胞中的表达及功能的研究进展[J].中国科学:生命科学,2021,51(12):1692-1700.
[3] Pereira da Silva EA, Martín-Aragón Baudel M, Navedo MF, et al. Ion channel molecular complexes in vascular smooth muscle[J]. Front Physiol, 2022, 13:999369.
[4] Alessandri-Haber N, Dina OA, Chen XJ, et al. TRPC1 and TRPC6 Channels Cooperate with TRPV4 to Mediate Mechanical Hyperalgesia and Nociceptor Sensitization[J].J Neurosci.2009,29(19):6217-28.
[5] Luo LH, Zhang YN, Chen HY, et al. Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells[J]. Cell Prolif. 2021, 54(2): e12969.
[6] Song Z, Chen L, Guo J, et al. The Role of Transient Receptor Potential Cation Channel, Subfamily C, Member 1 in the Odontoblast-like Differentiation of Human Dental Pulp Cells[J]. J Endod. 2017, 43(2):315-20.
[7] 王佳瑛,阮骏杰,张夏佳.口腔微生物在龋病的发病机制中的作用与防治的研究进展[J].浙江创伤外科,2025,30(10):1998-2002.
[8] Bleicher F. Odontoblast physiology [J]. Exp Cell Res, 2014, 325(2):65-71.
[9] Suzuki S, Sreenath T, Haruyama N, et al. Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization[J]. Matrix Biol. 2009, 28(4):221-9.
[2] 郭京,单徜,李玫,等.TRP离子通道在干细胞中的表达及功能的研究进展[J].中国科学:生命科学,2021,51(12):1692-1700.
[3] Pereira da Silva EA, Martín-Aragón Baudel M, Navedo MF, et al. Ion channel molecular complexes in vascular smooth muscle[J]. Front Physiol, 2022, 13:999369.
[4] Alessandri-Haber N, Dina OA, Chen XJ, et al. TRPC1 and TRPC6 Channels Cooperate with TRPV4 to Mediate Mechanical Hyperalgesia and Nociceptor Sensitization[J].J Neurosci.2009,29(19):6217-28.
[5] Luo LH, Zhang YN, Chen HY, et al. Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells[J]. Cell Prolif. 2021, 54(2): e12969.
[6] Song Z, Chen L, Guo J, et al. The Role of Transient Receptor Potential Cation Channel, Subfamily C, Member 1 in the Odontoblast-like Differentiation of Human Dental Pulp Cells[J]. J Endod. 2017, 43(2):315-20.
[7] 王佳瑛,阮骏杰,张夏佳.口腔微生物在龋病的发病机制中的作用与防治的研究进展[J].浙江创伤外科,2025,30(10):1998-2002.
[8] Bleicher F. Odontoblast physiology [J]. Exp Cell Res, 2014, 325(2):65-71.
[9] Suzuki S, Sreenath T, Haruyama N, et al. Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization[J]. Matrix Biol. 2009, 28(4):221-9.
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