125I粒子抑制肺腺癌细胞A549增殖的研究
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v7i2.13374
Abstract
目的：探究125I粒子对肺腺癌细胞A549增殖的抑制作用。方法：体外实验使用A549细胞分别接受125I粒子不同剂量的辐射刺激,具体分组如下：0cGy、400cGy和800cGy组。细胞照射完成后采用CCK8实验及细胞克隆形成实验方法检测细胞活力及细胞克隆能力。结果：CCK8实验结果显示,随着辐射剂量的加大,细胞活力均逐渐下降(与0cGy组相比,400cGy和800cGy组的细胞活力分别为84.194%±2.258和68.454%±5.465),差异有统计学意义；细胞克隆形成实验结果显示,随着辐射剂量的加大细胞克隆能力均逐渐下降(0cGy、400cGy和800cGy组分别对应的细胞克隆团数为134.667±8.737个、94.333±4.041个和60.333±5.508个),差异有统计学意义。结论：125I粒子抑制肺腺癌细胞的活力及克隆形成能力,从而发挥了抑制肺腺癌细胞生长的作用。
Keywords
125I粒子；肺腺癌；克隆形成实验；CCK8
Funding
河北省政府资助临床医学优秀人才项目；河北省医学科学研究课题计划项目(20210247；20221335)；2024年承德市应用技术与开发暨可持续发展议程创新示范区专项科技计划项目(202404B009)。
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