纳米孔测序在功能基因组学中的潜在应用回顾
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v6i5.10082
Abstract
分子生物学领域始终受到各种先进测量技术的推动,通量的不断提升为组学分析带来了飞跃性的发展。大规模并行测序技术的进步使得研究人员能够广泛获取分子数据,从而揭示基因组与转录组的深层特征。在这场技术革命中,纳米孔测序仪将测序能力推向了一个全新的高度。由Oxford Nanopore Technologies Inc.开发的纳米孔测序仪采用蛋白质纳米孔技术,能够直接读取单分子序列,而无需传统测序过程中常见的DNA合成或扩增步骤。该技术支持对超长核苷酸序列的测序,突破了传统技术对读长的限制,且还可以识别DNA或RNA修饰。近年来,这一技术在医学、流行病学、生态学以及教育等多个领域中取得了显著成果,并逐渐成为这些领域的核心工具。在这篇综述中,我们详细探讨了纳米孔测序仪的独特优势及其功能,阐述了该技术在各个领域中的具体应用实例,并展望了它在未来可能带来的更多突破与创新。
Keywords
长读长；纳米孔测序；下一代测序
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