基于网络药理学探讨紫草素对肝损伤的作用
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v6i4.8490
Abstract
目的：基于网络药理学方法探究紫草素改善肝损伤的分子作用机制。方法：运用Traditional Chinese Medicine Systems Pharmacology(TCMSP)、Pharm-Mapper检索 紫草素作用的相关靶点,并将检索得到的靶点在Uniprot蛋白质数据库进行标准化处理；通过OMIM、GeneCards数据库获取肝损伤的有效靶点；通过Venny平台,得到的交集靶点作为紫草素治疗肝损伤的靶点,运用Cytoscape3.7.0软件和String数据库构建紫草素-肝损伤交集靶点网络以及蛋白-蛋白相互作用(protein-protein interaction,PPI)网络图；采用DAVID数据库对关键靶点进行基因本体GO富集分析和京都基因与基因组百科全书(KEGG)富集分析,阐述紫草素改善肝损伤的作用通路及分子机制。结果：通过TCMSP和Pharm-Mapper数据库,获得289个紫草素靶点,从OMIM及GeneCards数据库中筛选出9707个肝损伤靶点,通过Venny平台找到252个紫草素与肝损伤的共表达靶点,在PPI网络图中得到关键靶点为AKT1,EGFR,ESR1,CASP3等10个核心靶点,通过GO富集和KEGG通路分析,主要涉及内分泌代谢通路、癌症通路、PI3K-Akt信号通路、Ras信号通及MAPK信号通路等。结论：紫草素可通过靶向AKT1,EGFR,ESR1,CASP3等多靶点和多途径的方式发挥改善肝损伤的作用。
Keywords
紫草素；肝损伤；网络药理学
Funding
新疆维吾尔自治区级大学生创新创业训练计划项目(No.S202213560017)。
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