不同细胞因子对人小胶质细胞中 LncRNA BISPR 表达影响
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v6i3.7510
Abstract
目的：探讨lncRNA BISPR在经不同干扰素及炎症因子处理后的人小胶质细胞HMC3中的表达变化。方法：RT-qPCR检测经IFN-γ(10 ng/ml)、TNF-α(10 ng/ml)、IFN-α2b(50 ng/ml)分别处理0、0.5、1、2、12、24h后的人小胶质细胞HMC3中lncRNA BISPR的表达水平,探讨BISPR对不同干扰素及炎症因子的应答情况。结果：BISPR表达均在IFN-γ、TNF-α、IFN-α2b处理24h后显著升高(P＜0.001,P＜0.001,P＜0.01)。结论：BISPR在HMC3中可被IFN-γ、TNF-α、IFN-α2b诱导表达,可能是小胶质细胞异常激活的关键靶点,参与了慢性神经退行性疾病的发生和发展。
Keywords
lncRNA BISPR；人小胶质细胞HMC3；炎症
Funding
承德医学院高层次人才科研启动基金(项目编号：202107)；河北省高等学校科学研究青年基金(项目编号：QN2023005)。
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