热胁迫下大岩桐花的转录组分析
Journal: Agricultural Science DOI: 10.12238/as.v5i3.2154
Abstract
试验以常温培养(WT)和高温胁迫(mut)的大岩桐花蕾为材料进行转录组学测序分析,WT组和mut组共有1885个差异表达基因,在胁迫响应、多生物过程以及温度响应等方面存在显著差异；与通路相关的差异表达基因主要富集在植物信号转导和内质网蛋白加工等方面；转录因子预测分析发现AP2/ERF类、bHLH、MYB-related、C2H2和NAC等转录因子在大岩桐响应高温胁迫时表达量较高,这些转录因子与植物激素信息转导和内质网蛋白加工有关的基因成为大岩桐响应高温胁迫的重要候选基因。
Keywords
大岩桐；高温胁迫；转录组；花发育
Funding
浙江省大学生创新创业训练计划(00004CS202013023039)；浙江省教育厅科研项目(00004C4Y201941297)。
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[1] 郭丽,朱飞雪,王存纲,等.温度与汞胁迫对大岩桐花粉萌发及花粉管生长的影响[J].分子植物育种,2022,20(2):511-517.
[2] Santiago J P, Sharkey T D. Pollen development at high temperature and role of carbon and nitrogen metabolites[J]. Plant Cell Environ. 2019,42(10):2759-2775.
[3] 宋姣敏,徐小萍,陈裕坤,等.热胁迫下水仙花叶片的转录组分析[J].热带作物学报,2019,40(10):1991-2000.
[4] 彭真.高温影响番茄花粉发育的细胞学研究及花药差异表达分析[D].浙江大学,2012.
[5] 于晓英,卢向阳,陈永华,等.瓜叶菊热胁迫下的基因差异表达[J].农业生物技术学报,2007(03):459-463.
[6] Jiao Y, Hu Q, Zhu Y, et al. Comparative transcriptomic analysis of the flower induction and development of the Lei bamboo (Phyllostachys violascens)[J]. BMC Bioinformatics. 2019,20(Suppl25):87.
[7] Quint M,Delker C, Franklin K A, et al. Molecular and genetic control of plant thermomorphogenesis[J].Nat Plants.2016,2:15190.
[8] Zhang G,Tang R,Niu S,et al.Heat-stress-induced sprouting and differential gene expression in growing potato tubers:Comparative transcriptomics with that induced by postharvest sprouting[J].Hortic Res.2021,8(1):226.
[9] Gross T,Becker A.Transcription Factor Action Orchestr ates the Complex Expression Pattern of CRABS CLAW in Arabidopsis[J].Genes (Basel).2021,12(11).
[10] Moyroud E,Kusters E,Monniaux M,et al.LEAFY blossoms [J].Trends Plant Sci.2010,15(6):346-352.
[11] Yamaguchi N.LEAFY,aPioneer Transcription Factor in Plants:AMini-Review[J]. Front Plant Sci.2021,12:701406.
[2] Santiago J P, Sharkey T D. Pollen development at high temperature and role of carbon and nitrogen metabolites[J]. Plant Cell Environ. 2019,42(10):2759-2775.
[3] 宋姣敏,徐小萍,陈裕坤,等.热胁迫下水仙花叶片的转录组分析[J].热带作物学报,2019,40(10):1991-2000.
[4] 彭真.高温影响番茄花粉发育的细胞学研究及花药差异表达分析[D].浙江大学,2012.
[5] 于晓英,卢向阳,陈永华,等.瓜叶菊热胁迫下的基因差异表达[J].农业生物技术学报,2007(03):459-463.
[6] Jiao Y, Hu Q, Zhu Y, et al. Comparative transcriptomic analysis of the flower induction and development of the Lei bamboo (Phyllostachys violascens)[J]. BMC Bioinformatics. 2019,20(Suppl25):87.
[7] Quint M,Delker C, Franklin K A, et al. Molecular and genetic control of plant thermomorphogenesis[J].Nat Plants.2016,2:15190.
[8] Zhang G,Tang R,Niu S,et al.Heat-stress-induced sprouting and differential gene expression in growing potato tubers:Comparative transcriptomics with that induced by postharvest sprouting[J].Hortic Res.2021,8(1):226.
[9] Gross T,Becker A.Transcription Factor Action Orchestr ates the Complex Expression Pattern of CRABS CLAW in Arabidopsis[J].Genes (Basel).2021,12(11).
[10] Moyroud E,Kusters E,Monniaux M,et al.LEAFY blossoms [J].Trends Plant Sci.2010,15(6):346-352.
[11] Yamaguchi N.LEAFY,aPioneer Transcription Factor in Plants:AMini-Review[J]. Front Plant Sci.2021,12:701406.
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