Research on the Anti-Tumor Mechanism of Artocarpus Lingnanensis Lectin
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v5i1.1805
Abstract
The purpose of this study was to investigate the mechanism of Artocarpus Lingnanensis Lectin (ALL) in inhibiting the growth of tumor cells EL-4. ALL was applied to mouse lymphoma cells EL-4, with PHA (100μg/mL) as a positive control drug. The toxicity of ALL (3.125, 6.25, 12.5, 25, 50, 100, 200μg/mL) on EL-4 cells was evaluated using the CCK-8 assay. Flow cytometry was used to assess the effect of ALL (25, 100 μg/ mL) on apoptosis and cell membrane potential of EL-4 cells. Western blotting was conducted to measure the protein expression levels of BAX, cleaved-Caspase-3, cleaved-Caspase-9, cleaved-PARP1. Fluorescence microscopy was employed to detect the concentration of free calcium ions. Results showed that compared to the control group, ALL had a significant inhibitory effect on EL-4 cells in a dose-dependent manner, with an IC50 value of 105.4μg/mL. Flow cytometry results indicated an increase in apoptosis rate of EL-4 cells with ALL treatment, leading to a higher proportion of depolarized mitochondrial membrane potential. Furthermore, the protein expression levels of BAX, cleaved-Caspase-3, cleaved-Caspase-9, and cleaved-PARP1 were significantly upregulated. In conclusion, ALL can promote apoptosis of EL-4 cells through the mitochondrial apoptosis pathway, inhibit their migration and invasion, and thereby exhibit anti-tumor effects.
Keywords
Artocarpus Lingnanensis Lectin, EL-4 cells, proliferation, mitochondrial apoptosis
Funding
Guangxi Higher Education Young Teachers’ Basic Ability Enhancement Project (Grant No. 2020KY07002), School-level Project of Guangxi University of Chinese Medicine (Grant No. 2017QN016)
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[14] Zepp M., Kovacheva M., Altankhuyag M., et al. IDK1 is a rat monoclonal antibody against hypoglycosylated bone sialoprotein with application as biomarker and therapeutic agent in breast cancer skeletal metastasis[J]. J. Pathol. Clin. Res., 2017, 4(1): 55-68.
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