The expression and clinical significance of Hook1 in colorectal cancer

Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v3i3.947

Lei Yue1, Yingchao Sun1, Yuqin Shen1, Kaiqi Lv2, Weiling Hu3

1. Department of Gastroenterology, Sir Run Run Shaw Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang, China
2. Shaoxing University School of Medicine, Shaoxing, Zhejiang, China
3. Department of Gastroenterology, Sir Run Run Shaw Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang, China Institute of Gastroenterology, Zhejiang University (IGZJU), Hangzhou, Zhejiang, China Zhejiang University Cancer Center, Hangzhou, Zhejiang, China


Purpose — Colorectal cancer is one of the most common gastrointestinal malignancies around the world. Early diagnosis of colon cancer is still a difficult problem for clinicians. This study aimed to determine the expression of Hook1 in colon cancer and evaluate its clinical role. Methods — Real-time Quantitative PCR was performed to characterize the difference of Hook1 mRNA expression between colon cancer specimens and normal colon specimens. Immunohistochemistry was used to evaluate the Hook1 expression in colon cancer tissue. Analyses of correlation between Hook1 level and clinicopathologic features and prognosis were also performed. Results — In total, 70 pair of surgical samples of patients with colorectal cancer were collected. And 70 patients were received postoperative following-up with the median follow-up being 38 months. Enhanced Hook1 mRNA levels were observed in colorectal cancer tissues (1.49%) compared to normal tissues (0.76%). IHC also revealed significantly higher rates of Hook1 expression amongst 58 cases in colon cancer tissues versus normal colon. Higher Hook1 protein levels were associated with better differentiation degree and easier likelihood of lymph node metastasis. Patients with relatively lower Hook1 level survived extended period without disease progression. And age, distant metastasis, TNM stage and Hook1 protein expression were marginally identified as poor prognosis indicator. Conclusion — Hook1 is highly expressed in colorectal cancer tissues, which correlate to well differentiated degree and lymph node metastasis tendency of colorectal cancer and potentially identified as a poor prognosis predictor.


Hook1, colorectal cancer, tumor biomarkers, differentiated degree, lymph node metastasis, prognosis


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