Design CRISPR-Cas9 Guide RNA and Donor Oligo to Introduce Pathogenic Mutation into Mice

Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v2i3.459

Xijing Liao1, Xueyan Yang2

1. YK Pao School, Shanghai 201620, China
2. School of Life Sciences, Fudan University, Shanghai 200433, China

Abstract

CRISPR-Ca9 system is a newly developed gene-editing technology, which is widely used in biology and medical research. In this project, we want to knock-in a mutation found in a human pedigree into mice through CRISPR-Cas9 technology to validate its pathogenic effect. We download corresponding mice genomic sequences and design guide RNA and donor oligo sequences according to CRISPR-Cas9 target principles. Following experiments confirm that this set of sequences is effective in mice cell line.

Keywords

CRISPR-Ca9, RNA, DNA sequences

References

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Copyright © 2021 Xijing Liao, Xueyan Yang

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