Study on Paeoniflorin Promoting the Recovery of Motor Function of Rat Spinal Cord Injury by Mediating MAPK/ERK and Akt/mTOR Signal Pathway

Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v2i1.302

Pei Zhang1, Nan Wu2, Zhijun Song3, Zhengfu Tai4

1. School of Chemical Engineering, Yunnan Open University
2. School of Medical, Yunnan University of Business Management
3. National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants
4. Sichuan Kelun Pharmaceutical Research Institute

Abstract

Spinal cord injury (SCI) is a devastating disease that can cause severe motor, sensory, and autonomic dysfunction. There is currently no effective treatment. Paeonia lactiflora is a traditional Chinese herbal medicine, which has antispasmodic, analgesic, and blood circulation effects. Paeoniflorin (PEF) is a medicinal plant isolated from Paeoniae Radio, and it is widely used in East Asia. A large number of studies have shown that PEF has a powerful neuroprotective effect. However, the potential mechanism of PEF on SCI needs further study. This project uses Basso Beattie Bresnahan (BBB) motor function score and open field test to evaluate neurological function, and uses immunofluorescence method to detect brain-derived neurotrophic factor (BNDF) and neurotrophin-3 (NT). -3) Protein expression, Western blot is used to detect protein expression level, and RT-PCR is used to detect mRNA expression level. Thus, a rat spinal cord injury model was established to observe the effects of AT/mTOR and MAPK/ERK signal pathways activated by PEF on nerve regeneration and functional recovery in rats with spinal cord injury.

Keywords

spinal cord injury, paeoniflorin, recovery

Funding

"Paeoniflorin promotes the recovery of motor function after spinal cord injury in rats" in Yunnan Open University (No.: 19YNOU05)

References

[1] Zhao H, Chen S, Gao K, et al. Resveratrol Protects Against Spinal Cord Injury by Activating Autophagy and Inhibiting Apoptosis Mediated by the SIRT1/AMPK Signaling Pathway. Neuroscience. 2017; (348): 241-251.
[2] Assinck P, Duncan GJ, Hilton BJ, Plemel JR, Tetzlaff W. Cell transplantation therapy for spinal cord injury. Nat Neurosci. 2017; (20): 637-647, .
[3] Thietje R and Hirschfeld S. Epidemiology of Spinal Cord Injury. In: Norbert Weidner, Rüdiger Rupp, Keith E. Tansey. Neurological Aspects of Spinal Cord Injury. Cham: Springer International Publishing Switzerland; 2017.
[4] Rodrigues LF and Moura-Neto V. Biomarkers in Spinal Cord Injury: from Prognosis to Treatment. Molecular Neurobiology. 2018; 1-13.
[5] Jalan D, Saini N, Zaidi M, Pallottie A, Elkabes S and Heary RF. Effects of early surgical decompression on functional and histological outcomes after severe experimental thoracic spinal cord injury. J Neurosurg Spine. 2017; (26): 62-75.
[6] Vasconcelos NL, Gomes ED, Oliveira EP, et al. Combing neuroprotective agents: effect of riluzole and magnesium in a rat model of thoracic spinal cord injury. Spine Journal Official Journal of the North American Spine Society. 2016; (16): 1015-1024.
[7] Sámano C and Nistri A. Mechanism of Neuroprotection Against Experimental Spinal Cord Injury by Riluzole or Methylprednisolone. Neurochemical Research. 2017; 1-14.
[8] Wu Q, Jing Y, Yuan X, et al. Melatonin treatment protects against acute spinal cord injury-induced disruption of blood spinal cord barrier in mice. Journal of Molecular Neuroscience. 2014; (54): 714-722.
[9] Heo SH, Lee DS, Ham SH, et al. Pharmacokinetic evaluation of paeoniflorin after oral administration of Paeoniae Radix extract powder to healthy Korean subjects using UPLC-MS/MS. Journal of Pharmaceutical Investigation. 2016; (46): 273-282.
[10] Salunga TL, Tabuchi Y, Takasaki I, et al. Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells. International Journal of Hyperthermia the Official Journal of European Society for Hyperthermic Oncology North American Hyperthermia Group. 2007; (23): 529-537.
[11] Kapoor S. Neuroprotective effects of paeoniflorin: an emerging concept in neurology. Folia Neuropathologica. 2013; (51): 92-92.
[12] Manayi A, Omidpanah S, Barreca D, et al. Neuroprotective effects of paeoniflorin in neurodegenerative diseases of the central nervous system. Phytochemistry Reviews. 2017; (16): 1-9.
[13] Zhang Y, Qiao L, Xu W, et al. Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca2+/CaMKII/CREB Signaling Pathway. Molecules. 2017; (22): 359.
[14] Feng Z, Sun Y, Liu R, et al. Neuroprotective mechanisms of Paeoniflorin on spinal cord injury in rats. Immunological Journal. 2016.
[15] Wang R, Peng X, Wang L, et al. Preparative purification of peoniflorin and albiflorin from peony rhizome using macroporous resin and medium-pressure liquid chromatography. Journal of Separation Science. 2012; (35): 1985-1992.
[16] Chen T, Guo ZP, Jiao XY, et al. Peoniflorin suppresses tumor necrosis factor-α induced chemokine production in human dermal microvascular endothelial cells by blocking nuclear factor-κB and ERK pathway. Archives of Dermatological Research. 2011; (303): 351-360.
[17] Wang X, Kong K, Shang J. A study on comparing open field test with combined behaviour score after thoracic spinal cord injury in rats. Journal of Shantou University Medical College. 2004; (17): 199-201.
[18] Martinez M, Brezun JM, Bonnier L, Xerri C. A new rating scale for open-field evaluation of behavioral recovery after cervical spinal cord injury in rats. Journal of Neurotrauma. 2009; (26): 1043-1053.

Copyright © 2021 Pei Zhang, Nan Wu, Zhijun Song, Zhengfu Tai

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License